For men undergoing an initial prostate biopsy how many biopsy cores, which pattern of biopsy sampling sites and which approach constitute an adequate prostate biopsy? (PICO questioni 7)
Guidelines developed in partnership with
Prostate Cancer Foundation of Australia and Cancer Council Australia PSA Testing Guidelines Expert Advisory Panel. Clinical practice guidelines PSA Testing and Early Management of Test-Detected Prostate Cancer. Sydney: Cancer Council Australia. [Version URL: http://wiki.cancer.org.au/australiawiki/index.php?oldid=122833, cited 2017 Nov 23]. Available from: http://wiki.cancer.org.au/australia/Guidelines:PSA_Testing/Biopsy_quality_criteria.
National Health and Medical Research Council
In approving the guidelines (recommendations), NHMRC considers that they meet the NHMRC standard for clinical practice guidelines. This approval is valid for a period of five years. NHMRC is satisfied that the guidelines (recommendations) are systematically derived, based on the identification and synthesis of the best available scientific evidence, and developed for health professionals practising in an Australian health care setting.This publication reflects the views of the authors and not necessarily the views of the Australian Government.
Core biopsy of the prostate with histological examination is indicated when investigations undertaken after the finding of raised PSA support the suspicion of prostate cancer (see 2.5 Testing with variants of PSA to improve sensitivity after an initial total PSA ≤ 3.0 ng/mL and 2.6 Testing with variants of PSA or repeat PSA testing to improve specificity after an initial total PSA > 3.0 ng/mL).
The purpose of core biopsy is to confirm the presence of cancer. If prostate cancer is confirmed, its type, grade and likely extent within the prostate is determined before definitive treatment is considered. A traditional approach was to collect a single core biopsy from six zones of the prostate (sextant biopsy). Current clinical practice varies considerably in the number of cores collected, with multiple cores taken from these six zones and extra cores directed at different areas of the prostate.
One systematic review, seven randomised controlled trials and 15 sequential sampling studies (three with sequential sampling in an intervention arm) were identified that provided evidence relevant to determining an optimal number of core biopsies, biopsy site, and surgical approach. From an initial 12,667 citations, 109 studies in 23 articles met inclusion criteria for the review (22 articles reporting one study each and one systematic review reporting data from 87 studies). The search strategy, inclusion and exclusion criteria, and quality assessment are described in detail in the Technical report.
The systematic review compared the cancer detection rates and complications of different extended prostate biopsy schemes for diagnostic evaluation in men scheduled for biopsy. It reported that ‘the standard sextant scheme has a significantly lower cancer yield than most of the more extensive biopsy schemes. As the number of cores increases, the yield improves for most of the schemes.’ However, the review did not determine an optimal biopsy number and did not disentangle the independent effects of increasing core numbers and biopsy location.
Studies published since the systematic review examined a diversity of proposed schemes and comparisons. We performed a patient-level regression analysis using data from nineteen additional studies that compared various biopsy protocols. Across the included studies, 23,822 biopsy components from 8,221 men were assessed for all cancers and 9,851 biopsy components from 3,701 men were assessed for cancers with Gleason score greater than 6.
Number of cores
For any given biopsy region or set of regions, men who had 24 cores taken had nearly double the odds of having cancer detected than men who had six cores taken (odds ratio [OR] 1.98; 95% confidence interval [CI] 1.52–2.58). There was also a clinically significant increase in cancer detection rate between 12 biopsies (45.6%) and 24 biopsies (56.9%) for populations in which the 6-core sextant scheme was predicted to yield 40%.
Evidence for adverse event rates was limited. It was not possible to compare rates of adverse events between groups who underwent biopsy with different numbers of cores.
Site of cores sampled
For a given number of cores, taking samples from the peripheral zones (i.e. the lateral peripheral zone [LPZ] and/or the mid-peripheral zone [MPZ]) yielded more cancers than taking samples from the transitional zone. The relative increases in yield from increasing core numbers was similar for higher-grade (Gleason score > 6) cancers and all cancers. Overall, the evidence did not show that, for a given number of cores, sampling regions in addition to the peripheral zones (i.e. LPZ and/or MPZ) led to increases in cancer yield.
Evidence for adverse event rates was limited. It was not possible to compare rates of adverse events between groups who underwent biopsy with different sampling sites.
There was insufficient evidence to determine if the transperineal approach was superior to the transrectal approach for cancer detection. None of the included studies measured concordance between biopsy and post-prostatectomy histopathology in individual patients.
Two studies directly compared adverse events in men who underwent 12-core biopsy using the transperineal and transrectal approaches. In one study, the perineal approach was associated with a significantly higher rate of headaches. Neither reported differences in other adverse events, including fever and sepsis (reported in one study). Neither study reported infection rates.
Evidence summary and recommendations
| Detection of prostate cancer
Increasing biopsy core number improves cancer yield; as the number of cores increases, the yield increases. A patient-level regression analysis showed that:
For a given number of cores, taking samples from the peripheral zones (i.e. LPZ and/or MPZ) yielded more cancers than the transitional zone.
|I||, , , , , , , , , , , , , , , , , , , |
| Detection of prostate cancer
There is insufficient evidence to determine if the transperineal approach is superior to the transrectal approach in detecting cancer.
|I||, , , , , , , , , , , , , , , , , , |
| Detection of cancer with Gleason score > 6
The relative increases in yield from increasing core numbers was similar for higher-grade cancers (Gleason score > 6) and all cancers.
Overall, the evidence did not show that, for a given number of cores, sampling regions in addition to the peripheral zones (i.e. LPZ and/or MPZ) led to either an increase or a decrease in yield of cancers with Gleason score > 6.
There is insufficient evidence to determine if the transperineal approach is superior to the transrectal approach in detecting cancers with Gleason score > 6.
|I||, , , , , |
| Adverse events
Evidence on adverse events is limited.
Differences in adverse event rates were not consistently associated with the number of core biopsies or with the biopsy pattern.
|II||, , , , , , |
| Adverse events
There is insufficient evidence to determine whether the transperineal approach is consistently associated with a lower rate of adverse events than the transrectal approach.
|Take 21–24 cores in initial biopsies for the diagnosis of prostate cancer. In addition to the sextant biopsies, direct 15–18 additional biopsies to the peripheral zones of the prostate.||B|
Before offering biopsy after an elevated total PSA test result, take into account a man’s family history of prostate cancer (see Chapter 1 Risk) and the results of further investigations (see 2.5 Testing with variants of PSA to improve sensitivity after an initial total PSA ≤ 3.0 ng/mL and 2.6 Testing with variants of PSA or repeat PSA testing to improve specificity after an initial total PSA > 3.0 ng/mL).
Transrectal and transperineal biopsy approaches are both acceptable with respect to rates of cancer detection. The approach taken should be based on the man’s wishes, the surgeon’s experience, risk of sepsis and other morbidity, and practical issues such as cost and access to the necessary facilities.
Health system implications
While the recommendation has already been adopted by some urologists, some routinely collect fewer biopsy samples. Accordingly, implementation of the recommendation would result in an increased number of core biopsies per patient, which could increase morbidity and infection rates.
Implementation of this recommendation may result in prostate biopsy becoming a procedure that is mainly performed in operating theatres and with general anaesthesia.
Implementation of this recommendation would result in a small increase in the time needed to perform biopsies and a modest increase in pathology costs. No changes in equipment would be needed unless transperineal biopsy with template is considered.
Barriers to implementation
No barriers to the implementation of this recommendation are envisaged.
i Clinical questions were translated into the PICO framework: population, intervention (or exposure), comparator and outcome (see Appendix 3).
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